Bioinformatics: Tutorials & Lessons: Using ClustalW to do a multiple sequence alignment
ADVERTISEMENTS
Bioinformatics: Tutorials & Lessons: Using ClustalW to do a multiple sequence alignment
In this video tutorial i'll be showing how to use ClustalW program to do a multiple sequence alignment.
You can read about multiple sequence alignment and ClustalW program in this post HERE.
If you want more informations about main multiple sequence alignment applications, you can read this post HERE.
Posts
2 comments:
How do I get the alignment shown at the end of this video into a format that is useful in the PHYLIP program? If I just copy and paste the CLUSTAL 2.1 multiple sequence alignment that it gives me, the PHYLIP program won't work. How do I get my aligned sequences to look like the ones you used in your video on PHYLIP? If you could e-mail me an answer that would be greatly appreciated! jguter@middlebury.edu
Thanks you!
Hello,
1- You can choose the Phylip format as an output before you do the alignment with ClustalW. This format is the one compatible with the Phylogeny inference package. In the "output" drop down menu, select Phylip as there are normally five formats that ClustalW exports to.
2- The other solution is to convert your existing format to Phylip format. You can just Google this phrase: ClustalW to Phylip and you'll find scripts where you can paste your format and convert it to Phylip format.
When you get the Phylip format, you can use it to construct phylogenetic trees as i did in my video tutorial.
Post a Comment