Bioinformatics: Different Blast Programs

Bioinformatics: Different Blast Programs

BLAST or (Basic Local Alignment Search Tool) is a set of programs that search for similar sequences to your query sequence, so you can find hundreds of similar sequences to yours in about 20 seconds.

Blast have a set of programs, each with a specific role:

BLASTN: Nucleotide query sequence against nucleotide sequence database.

BLASTP: Amino acid query sequence against a protein sequence database. you can find it HERE.

BLASTX: Nucleotide query sequence translated in all six reading frames against a protein sequence database.

TBLASTX: Six-frame translations of a nucleotide query sequence against the six-frame translations of a nucleotide sequence database.

TBLASTN: Protein query sequence against a nucleotide sequence database translated in all six reading frames. you can find it HERE.

Or you can find them all at ch.EMBnet.org

You can find also other programs such as:

1- PSI-BLAST: Position Specific Iterative BLAST detects weak homologs by building a profile from a multiple alignment of the highest scoring hits in an initial BLAST search.
Available at NCBI .

2- PHI-BLAST: Pattern-Hit Initiated BLAST combines matching of regular expressions with local alignments surrounding the match.
Available at NCBI .

To learn how to use Blast to search for similarities, you can see this Video Tutorial HERE.

Any questions, you are welcome.